ClaricepTMFlash spherical products use silica with a narrow particle size distribution, which has high column efficiency. Bonna Agela has developed a series of inverted and normal phase spherical Flash columns. Reverse phase includes C18, AQ C18 column, C18 (2), Phenyl, C4, C8, and HLP, while positive phase includes CS, CM, and amide (HILIC).
ClaricepTMFlash spherical C18 column, 20-35 μ m, 100 Å, carbon content 14%.
Chromatographic column: ClaricepTMFlash C18100 Å, 20-35 μ m, 80g, two in series;
Mobile phase: A： Acetonitrile; B： Water;
Wavelength: 280 nm;
Detector: UV 254 nm，280 nm；
Flow rate: 60 mL/min;
Injection volume: 5 mL;
Chromatographic column: Competitive brand, 40-60 μ m, 80 g, two in series;
Mobile phase: A： Acetonitrile; B： Water;
Wavelength: 280 nm;
Detector: UV 254 nm，280 nm；
Flow rate: 60 mL/min;
Injection volume: 5 mL;
Conclusion: From the above experimental analysis, it can be concluded that the separation effect of 20-35 μ m is better and suitable for preparing samples with higher purity. Small particle size fillers have better peak shapes and separation effects.
Sample: Paclitaxel
Chromatography column: ClaricePTM Flash AQ C18100 Å, 20-35 μ m, 120 g;
Mobile phase: A： Water; B： Methanol;
Wavelength: UV 230 nm、254 nm；
Flow rate: 83 mL/min;
Injection volume: 20 mL
Gradient:
Sample: A certain peptide
Chromatographic column: ClaricePTM Flash C18 (2), 100 Å, 20-35 μ m, 800 g;
Mobile phase: A: 0.1% TFA aqueous solution; B： Acetonitrile;
Wavelength: UV 214 nm、254 nm；
Flow rate: 160 mL/min;
Injection volume: 8 g
Gradient:
